class: center, middle, inverse, title-slide # Research Process ## (面向低年级本科生的)简明介绍 ###
Dongliang Xiong | 熊栋梁
https://dlxiong.org
### 2019/07/20 --- ## How to do (scientific) research? <img src="Images/ResearchProcess.png" width="52%" style="display: block; margin: auto;" /> --- ## Why do I need a research question? - A research question helps keep your research focused and on track. - The answer to this question will turn into your thesis statement. <img src="Images/01-phd.jpg" width="50%" style="display: block; margin: auto;" /> --- ## How do I come up with a good research question? 1. Find an issue that interests you 2. Explore the issue <img src="Images/02-GoogleSchoolar.png" width="82%" style="display: block; margin: auto;" /> How to find papers? go to my previous [**blog**](https://www.dlxiong.org/presentation/2019-03-31_findpapers.html#1) --- ## How do I come up with a good research question? 1. Find an issue that interests you 2. Explore the issue - Articles (SCI) - Books (TextBook) - Discussion - WiKipedia (NO Baidu Baike) - ... 3. Start asking questions 4. Refine and focus your question --- ## What are the features of a good research question? 1. The question is actually a question 2. Short 3. Clear 4. Not premised on an assumption 5. Can NOT be answered as a yes/no .pull-left[ - Good questions - 水稻光合作用是怎么响应CO<sub>2</sub>升高的? - 夜间增温影响水稻产量的机理是什么? - 水分是如何从土壤运输到叶片的? ] .pull-right[ - Bad questions - 作物水分利用效率 - 高温影响水稻产量吗? - 光合作用如何响应环境条件变化? ] --- ## Formulate hypotheses The research hypothesis is a statment that is testable with research data. - What kind of sources (traits) will you need to answer your question? - Why does your argument matter? - How might others challenge your argument? <img src="Images/collaboration.jpg" width="100%" style="display: block; margin: auto;" /> --- ## Formulate hypotheses For your specific research question, you may have a banch of hypotheses. <center><span style = "color:blue"><font size="5.0"> Hypothesis Test </font></span></center> <img src="Images/03-HypothesisTests.jpg" width="60%" style="display: block; margin: auto;" /> --- ## Why do I need a Research Proposal - To give a concise and clear outline of the objectives that you want to achieve through your project - To serve as a **planning tool** - To present it to Educational institutions - To apply for funding/position --- ## Research Proposal Format - **Brief introduction** - **Research question** - **Theoretical background** (fomating question and hypothesis processes) - Literature review: A critical review of the theories, concepts, debates and major viewpoints around your research question - Specific emphasis to how you rise the research question - Assumptions - Concepts - Relationships - **Hypotheses and/or Objectives** - Null hypothesis - Alternative hypothesis - Relationships --- ## Research Proposal Format - **Proposed research methodology** - The context/ setting of the study (research design) - Sample size and other characteristics - Method of data collection - Quantitative and/or qualitative - survey, interviews, archival data etc - measurement of the concepts/ variables - independent variable - dependent varibale - intervening varibale --- ## Some tips for proposal development - Read papers, books and relative materials as many as you can - Discuss with your team members as often as possible - Meet your mentor - Cool down the script (weeks) and review it <img src="Images/04-proposal.jpg" width="60%" style="display: block; margin: auto;" /> --- ## <span style = "color:blue"> Data collection</span> _**The footstone of modern biology research**_ ### <span style = 'color:red'> Types of data </span> - Primary data - Experiment (Obervation) - Survey (interview, questionnaires, schedules et al.,) - Secondary data - Systhesis analysis - Meta-analysis --- ## <span style = "color:blue"> Data collection</span> - **Repeatability** (for yourself and your group) - _**Reproducibility**_ (for everyone) <img src="Images/05-notebook.jpg" width="60%" style="display: block; margin: auto;" /> <span style = "color:blue"><font face = 'bold'>How to organize your research notes? </font></span> Please read Prof. Xiong's [slides](http://croplab.hzau.edu.cn/__local/E/E2/B3/278D1B2DF2CDCF23C793EA9BD95_1A0BED1E_31F61.pdf?e=.pdf) --- ## <span style = "color:red"> Data analsysis</span> <img src="Images/06-dataanalysis.png" width="80%" style="display: block; margin: auto;" /> --- ## <span style = "color:red"> Data analsysis</span> - **Managing your Project** <img src="Images/07-clusterdesck.png" width="120%" style="display: block; margin: auto;" /> - Manage large amounts of various data files. - Distinguish different files and versions of files within a folder. - Prevent confusion when working on teams or sharing files. - Prevent data loss by accidentally overwriting or deleting files. - Provide context for retrieval and storage of data. - ... --- ## <span style = "color:red"> Data analsysis</span> - **Managing your Project** .pull-left[ <img src="Images/08-filemanagement.png" style="display: block; margin: auto auto auto 0;" /> ] .pull-right[ <img src="Images/09-orgnization.png" style="display: block; margin: auto auto auto 0;" /> ] --- .pull-left[ **As chief identifier ** - Keep the filename short (~25) - Use consistent elements - Standard format for dates. - Avoid special characters - leading zeros (e.g. 001) - Don’t overwrite file extensions ] .pull-right[ **Make sense to everyone** - Project name - Name of file creator - Sequence ID - Accession number - Date of creation - Version number ] <img src="Images/10-filename.png" style="display: block; margin: auto;" /> --- ## <span style = "color:red"> Data analsysis</span> <img src="Images/11-dataSworkflow1.png" style="display: block; margin: auto;" /> --- ## <span style = "color:red"> Data analsysis</span> <img src="Images/12-leaftraits.png" style="display: block; margin: auto;" /> --- ## <span style = "color:red"> Data analsysis</span> <img src="Images/kid-waves.gif" width="200%" style="display: block; margin: auto;" /> --- ## <span style = "color:red"> Data analsysis</span> <img src="Images/13-tidyverse.png" style="display: block; margin: auto;" /> --- ## <span style = "color:red"> Data analsysis</span> <img src="Images/14-researproRGit.png" width="90%" style="display: block; margin: auto;" /> --- ## <span style = "color:red"> Data analsysis</span> **What do I need** - R (https://www.r-project.org/) - Rstudio (https://www.rstudio.com/) - Repository service, i.e. dropbox, box, drive,Gitlab, Github et al., - LaTex tools i.e. Overleaf (or simply using PanDoc) <img src="Images/15-Rrelatives.png" style="display: block; margin: auto;" /> --- ## <span style = "color:DarkMagenta"> Outputs</span> <img src="Images/16-writting.png" width="90%" style="display: block; margin: auto;" /> --- ## <span style = "color:DarkMagenta"> Outputs</span> **Submission** - Target Journal (IF, OA, Speed et al.,) - Format - Cover letter - Editors and/or Reviewers - Data access - ... --- ## <span style = "color:DarkMagenta"> Outputs</span> **Peer review** <img src="Images/17-submission.jpg" width="80%" style="display: block; margin: auto;" /> --- <img src="Images/18-giphy.gif" width="90%" style="display: block; margin: auto;" /> <br><br> <center> <span style = "color:blue"><font size="6.0"; face="bold"> Enjoy your trip ! </span></font> <br> Email: dlxiong@mail.hzau.edu.cn <br> Homepage: https://www.dlxiong.org </center>